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Table 4 Reduction of random integration by S-phase synchronization

From: Gene targeting in adult rhesus macaque fibroblasts

Targeting Vector

S-phase synchronization

# of cells

G418rcolonies

pHPRT+/-

No

107 cells

564

pHPRT+/-

Yes

107 cells

20

  1. The targeting vector used in this experiment did not contain the SV40 enhancer.
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BMC Biotechnology

ISSN: 1472-6750

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