Table 2 Results of conventional HPRT targeting strategies in hTERT transfected fibroblasts
Vector | Promoter | IRES | Antibiotic | Coloniesr/5 × 106 cells | Targeting events |
|---|---|---|---|---|---|
pMK HPRT INEO | None | Yes | NEO | 0 | 0 |
pMK HPRT IPURO | None | Yes | PURO | 0 | 0 |
pHPRT+/- | PGK | No | NEO | 282 | 0 |