Figure 3
RT-PCR for TERT expression in rhesus fibroblast cells. a) CDS = coding region, T2A = hTERT-T2A, T2B = hTERT-T2B, ENF3 = MK TERT ENF3, ENR3 = MK TERT ENR3, blue line = portions of TERT included in pCI-neo-hTERT construct. b) M = 100 bp marker; WT = wild type rhesus fibroblasts; ES = rhesus embryonic stem cells; TTF = TERT transfected fibroblasts (experiment #8, HPRT null clone) T3' = Amplicons of 3' UTR TERT found in endogenous TERT mRNA but not present in construct hTERT mRNA; TC = Amplicons of coding TERT found in both endogenous and construct hTERT mRNA.