Figure 1

Production of IPNS by H. polymorpha strain IPNS 4.2 cultivated at various temperatures. Western blots showing IPNS production in cells of the H. polymorpha strain IPNS 4.2 cultivated at 37°C (panels A and B), 30°C (panels C and D) and 25°C (panels E and F). Aliquots were taken at different stages during cultivation at approximately equal optical densities (expressed as A₆₆₀ and indicated as 1, 2, 3, and 4 in panels A, C and E). IPNS is produced in strain IPNS 4.2 at all growth temperatures. However, during later stages of growth relatively low IPNS protein levels were observed in cells grown at 37°C, while at 30°C and 25°C enhanced levels of IPNS protein were detected.