Figure 4
From: Design of a zinc finger protein binding a sequence upstream of the A20 gene
A: RT-PCR was performed on total RNA isolated from COS7 cells transiently transfected for 24 h with pIRES2-EGFP/ZFP-flag. Agarose gel electrophoresis indicated an obvious specific amplification band at 392 bp. B: Western blotting. The pIRES2-EGFP/ZFP-flag transfection group showed clear protein bands 48 h after transfection, but these did not appear in the pIRES2-EGFP control group. C: Electrophoretic mobility shift assay. Lane 1: sample reaction array. Lane 2: "cold" competition assay of probes array. Lane 3: "cold" competition assay of mutated probes array. Lane 4: negative control reaction array. Lane 5: Super-Shift reaction array. A lag band of "antigen-antibody-specific probe complex" was observed (indicated by arrow).