TY - JOUR AU - Wei, Yong AU - Ying, Dajun AU - Hou, Chunli AU - Cui, Xiaoping AU - Zhu, Chuhong PY - 2008 DA - 2008/03/19 TI - Design of a zinc finger protein binding a sequence upstream of the A20 gene JO - BMC Biotechnology SP - 28 VL - 8 IS - 1 AB - Artificial transcription factors (ATFs) are composed of DNA-binding and functional domains. These domains can be fused together to create proteins that can bind a chosen DNA sequence. To construct a valid ATF, it is necessary to design suitable DNA-binding and functional domains. The Cys2-His2 zinc finger motif is the ideal structural scaffold on which to construct a sequence-specific protein. A20 is a cytoplasmic zinc finger protein that inhibits nuclear factor kappa-B activity and tumor necrosis factor (TNF)-mediated programmed cell death. A20 has been shown to prevent TNF-induced cytotoxicity in a variety of cell types including fibroblasts, B lymphocytes, WEHI 164 cells, NIH 3T3 cells and endothelial cells. SN - 1472-6750 UR - https://doi.org/10.1186/1472-6750-8-28 DO - 10.1186/1472-6750-8-28 ID - Wei2008 ER -