Figure 4

Gel-electrophoretic analysis of a two-step elution in column 1. Column 1 was loaded with soybean feedstock mixture (705 μg total protein, 77% of which are P34) in initial binding buffer (1 M (NH₄)₂SO₄ in 0.05 M phosphate buffer, pH 6.5). Elution was performed at 0.6 M and 0.4 M (NH₄)₂SO₄. In the Coomassie-stained polyacrylamide gel, one fraction is represented by one lane: lanes 1 – 4 show the flow-through in binding buffer, lanes 5 – 12 the fractions eluted with 0.6 M (NH₄)₂SO₄ and lanes 13 – 21 the fractions eluted with 0.4 M (NH₄)₂SO₄ and lanes 22 29 represent the fractions eluted in phosphate buffer during the washing step.