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Figure 3 | BMC Biotechnology

Figure 3

From: Isolation of soybean protein P34 from oil bodies using hydrophobic interaction chromatography

Figure 3

Gel-electrophoretic analysis of flow-through and elution for six differently functionalised HIC resins. Fractions from different resins, as described in the Methods section, were precipitated and separated using gel electrophoresis; from each column, two lanes (eluted proteins in binding buffer (flow-through) and at 0.4 M (NH4)2SO4) of every gel were included into this figure. P34 is represented by a band at ~32 kDa. Lane 0 represents the soybean feedstock used as column feed (diluted to 1 μg of total protein), lanes 1 and 2 are eluted fractions collected from Butyl Sepharose 4 Fast Flow, lanes 3 and 4 from Butyl-S Sepharose 6 Fast Flow, lane 5 und 6 from Octyl Sepharose 4 Fast Flow, lane 7 and 8 from Phenyl Sepharose 6 Fast Flow (low sub), lane 9 and 10 from Phenyl Sepharose 6 Fast Flow (high sub) and 11 and 12 from Phenyl Sepharose High Performance.

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