Comparison of different real-time PCR chemistries and their suitability for detection and quantification of genetically modified organisms

Table 6 Comparison of general applicability and practicability of the designed assays

	Modified probes			Modified primers
Investigated characteristic	TaqMan^®^a	LNA^®	CPT	Lux™	Plexor™
Labour intensity to design	low	low	middle	high	middle
Primer/probe design	software	on demand	on demand	software	software
Redesigns required (No. of unsuccessful/successful designs)	-	0/2	1/2	3/4	1/4
Price to establish a new system	high	high	very high	middle	middle
Price for 100 × 10 μl reactions in Euros^a	46	48	185	50	42
Run duration (minutes)	120	120	84	144	87
Labour intensity to analyze	low	low	low	middle	middle

^a Well established TaqMan^® based amplicons were analyzed in parallel as a reference system. ^b Estimated costs include PCR reagent, primers and probes but exclude plastics and optical covers since they are the same for all tests. Prices are stated for Slovenia, European Union, and may differ in other countries.

ISSN: 1472-6750