Skip to main content

Table 5 Accuracy of quantification performed by different chemistries

From: Comparison of different real-time PCR chemistries and their suitability for detection and quantification of genetically modified organisms

 

100% MON 810

0.29% MON 810

Detection chemistry

determined GMO content (%)

determined GMO content (%)

z-value

TaqMan®a

73 ± 6

0.24 ± 0.15

0.19

LNA®

95 ± 10

0.12 ± 0.05

-0.91

CPT

51 ± 16

0.28 ± 0.09

0.43

Lux™

65 ± 34

0.45 ± 0.45

0.37

Plexor™

50 ± 6

0.27 ± 0.09

1.18

  1. a Well established TaqMan® based amplicons were analyzed in parallel as a reference system.