Table 3 Performance of different chemistries, compared as LOD, LOQ, efficiency, dynamic range and repeatability
No. of copies | TaqMan®c | LNA® | CPT | Lux™ | Plexor™ | |||||
|---|---|---|---|---|---|---|---|---|---|---|
Invertase | ||||||||||
40000 | 24.07 (6) | 23.84 (6) | 23.15 (14) | 23.00 (6) | 24.74 (4) | 25.13 (11) | 26.76 (4) | 26.69 (2) | 24.41 (11) | 24.57 (8) |
4000 | 27.57 (6) | 27.31 (3) | 26.82 (9) | 26.52 (8) | 28.25 (13) | 28.21 (13) | 30.51 (11) | 30.23 (8) | 27.96 (11) | 28.13 (7) |
400 | 30.94 (10) | 30.68 (8) | 30.14 (19) | 29.93 (9) | 31.98 (13) | 31.91 (23) | 34.33 (15) | 34.07 (9) | 31.44 (11) | 31.61 (14) |
200 | 32.09 (6) | 31.62 (3) | 31.06 (8) | 30.83 (7) | 33.09 (17) | 32.16 (25) | 34.82 (12) | 34.70 (22) | 32.63 (5) | 32.51 (21) |
100 | 33.00 (15) | 32.63 (13) | 32.24 (18) | 31.80 (8) | 33.25 (26) | 33.75 (49) | 35.71 (39) | 35.59 (25) | 33.46 (24) | 34.00 (14) |
20 | 35.85 (43) | 35.28 (15) | 34.31 (19) | 34.16 (32) | 38.89 (124) | 4/5 | 3/5 | 3/5 | 35.72 (35) | 35.90 (41) |
4 | 4/5a | 4/5 | 2/5 | 1/5 | 4/5 | 1/5 | 2/5 | 1/5 | 4/5 | 3/5 |
0.4 | 1/5 a | 2/5 | 0/5 | 1/5 | 1/5 | 0/5 | 0/5 | 0/5 | 1/5 | 0/5 |
MON 810 | ||||||||||
8000 | 25.23 (2)b | 24.95 (5) | 24.82 (4)b | 24.55 (3)b | 23.38 (3)b | 24.40 (3)b | 28.78 (6)b | 28.76 (6)b | 26.96 (9) | 25.42 (11) |
4000 | 26.00 (3) | 25.92 (2) | 25.41 (5) | 25.34 (3) | 24.11 (7) | 25.17 (3) | 29.59 (4) | 29.59 (5) | 27.94 (5) | 26.58 (16) |
400 | 29.41 (15) | 29.14 (8) | 28.56 (9) | 28.53 (4) | 27.42 (5) | 28.38 (4) | 32.82 (14) | 32.95 (7) | 31.37 (5) | 30.49 (15) |
200 | 30.57 (12) | 30.24 (10) | 29.66 (13) | 29.57 (17) | 28.67 (6) | 29.44 (2) | 34.23 (13) | 33.98 (18) | 32.53 (7) | 31.60 (14) |
100 | 31.54 (13) | 31.38 (24) | 30.81 (4) | 30.53 (12) | 29.63 (7) | 30.43 (8) | 35.33 (19) | 35.14 (11) | 33.71 (18) | 32.65 (19) |
20 | 33.94 (31) | 34.06 (47) | 33.19 (11) | 32.74 (17) | 32.46 (44) | 33.47 (26) | 38.61 (73) | 37.62 (35) | 36.25 (19) | 34.86 (28) |
4 | 4/5 | 36.60 (44) | 4/5 | 3/5 | 4/5 | 2/5 | 3/5 | 39.45 (50) | 4/5 | 2/5 |
0.4 | 2/5 | 1/5 | 0/5 | 1/5 | 1/5 | 0/5 | 1/5 | 2/5 | 0/5 | 0/5 |