Figure 7
From: Effects of size and topology of DNA molecules on intracellular delivery with non-viral gene carriers
Transfection efficiency of c-DNA, l-DNA, and pcr-DNA using PEI as the gene carrier in HEK 293T Cells (A and B) and rBMSC (C and D). Cells were grown in 12-well plates and incubated with the complexes for 24 hours. EGFP expression was assessed on Day 1 and 4 for 293 cells, and on Day 3 for rBMSC. Results from the flow cytometry were expressed as either (i) percentage of EGFP-positive cells, when control cells were assigned to ~1% EGFP-positive cells (A and C), or (ii) the relative fluorescence values for EGFP-positive cells (B and D). The control cells in both cases were the cells exposed to PEI/gWiz-blank complexes without the EGFP gene. In the case of 293 cells, the relative fluorescence for the EGFP-positive cells was normalized with the fluorescence values for control cells to account for the day-to-day differences. For rBMSC, the mean fluorescence values were shown without normalization.