Figure 6
From: Effects of size and topology of DNA molecules on intracellular delivery with non-viral gene carriers
DNA delivery by PEI (a and e), PLL (b and f), PLL-PA (c and g), and Lipofectamine-2000™ (d and h) in the presence and absence of serum. The cells were grown in 24-well plates for 24 hours, and incubated with carrier/DNA complexes for an additional 4 and 24 hours. Results from the flow cytometry were expressed either as percentages of cells positive for Cy5.5-fluorescence (a, b, c and d) or mean fluorescence for Cy5.5-positive cells (e, f, g and h). Statistical differences between the medium with and without serum are indicated above: *p < 0.05, **<0.025, ***p < 0.01 and ****p < 0.001.