Figure 5
From: Effects of size and topology of DNA molecules on intracellular delivery with non-viral gene carriers
Delivery of c-DNA, l-DNA and pcr-DNA into rBMSC using PEI (a), PLL (b), PLL-PA (c), and Lipofectamine-2000™ (d) at various concentrations of carriers. The cells were grown in 6-well plates for 24 hours, and incubated with carrier/DNA complexes for an additional 24 hours. Results from the flow cytometry were expressed as percentages of cells positive for Cy5.5-fluorescence, where the cells incubated with DNA alone (i.e., without any carrier) was calibrated at ~1%.