Skip to main content
Figure 1 | BMC Biotechnology

Figure 1

From: Effects of size and topology of DNA molecules on intracellular delivery with non-viral gene carriers

Figure 1

Structure of the DNA molecules used in this study. A. Structure of the parent c-DNA. B. l-DNA formed by restriction digest of c-DNA with the enzyme XhoI. The size of the linearized plasmid was 4272 bp, C. pcr-DNA (2209 bp) generated by site-specific primers that amplified the region of the c-DNA containing the HTLV promoter, the hOPG open reading frame, and the SV40 Poly-A site. D. Structure of the parent c-DNA used in transfection. E. l-DNA formed by digest of pEGFP-N2 with the enzyme ClaI, which cuts in the vector backbone. F. pcr-DNA (1713 bp) generated by primers that are complementary to the sequence upstream of the CMV promoter and downstream of the polyA site.

Back to article page