Figure 2

Schematics of the retroviral vectors. Standard retroviral vectors were based on the pC-GFP vector [12]. pC-cGreen and pC-neerGc, carry an additional optimized GFP expression cassette comprising the CMV immediate early promoter, a chimeric intron and SV40 late pA, in opposite orientations. The pT-GFP vector has a T7RU5 composite 5'-LTR and modified 3'LTR where the U5 region has been replaced by a poly-A tract for in vitro transcription of vector genomic RNA [9]. pT-cGreen and pT-neerGc each contain a CMV expression cassette as above.