Figure 6

Detection of the deletion-circle as a result of Cre-mediated recombination. (A) An example of the four observed peaks in an adult tamoxifen treated Cre; Pkd1^{del2-11, lox} mouse, three peaks corresponding to the Pkd1^lox and Pkd1^del2-11 alleles plus an additional 144 bp peak. (B) The possible locations of probes B and C on the Pkd1^lox allele and on the deletion-circle. Probes B and C can only form a product when the floxed fragment is excised and circularized by Cre. (C) Hybridization of probes B and C followed by extension, ligation, 36 cycles of amplification and agarose-gel-electrophoresis does not result in a product when DNA from two Pkd1^{del2-11, lox} mice was used, but when DNA from kidneys from two adult tamoxifen treated Cre; Pkd1^{del2-11, lox} mice was used as a template, a distinct product of 144 bp was observed.