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Figure 3 | BMC Biotechnology

Figure 3

From: Quantification of Cre-mediated recombination by a novel strategy reveals a stable extra-chromosomal deletion-circle in mice

Figure 3

Outline for calculating the percentage of deletion allele. The percentage of Pkd1del2-11 for a sample s1 is used as an example. (A) The peak-ratios (P) for all controls and sample s1 is calculated by dividing the height of the deletion peak with the sum of the lox peaks. (B) A fifty percent reference (Ref.) is calculated by taking the median of the peak-ratios from all controls. (C) The peak-ratio for sample 1 (Ps1) is divided by and thus normalized to the fifty percent reference. The ratio of the Pkd1del2-11 allele to the Pkd1lox allele in sample 1 is, Rs1 to 1. (D) Thus, the fraction of the Pkd1del2-11 allele relative to the sum of the Pkd1del2-11 and Pkd1lox alleles is Rs1 divided by Rs1 plus 1. The total percentage of Pkd1del2-11 in sample 1 (Ds1) can now be calculated by multiplying this fraction with the maximum percentage (M) the Pkd1del2-11 and Pkd1lox alleles can have together. M is either 50% when initially one Pkd1lox and one Pkd1+ allele are present or a 100% when both alleles were either Pkd1lox or Pkd1del2-11.

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