Figure 1

The eMLPA strategy. (A) The position of the probes A, B, C, and D in case of the Pkd1^lox allele or the Pkd1^del2-11 allele after hybridization. (B) The gap between probes is filled in using Stoffel-Taq Polymerase. (C) The remaining nick is ligated by ligase, completing the formation of the templates AB, CD, and AD which are amplified simultaneously in a PCR reaction containing a fluorescently labeled forward primer.