Figure 2
Amplification of multiple genomic loci from single cells. (A) Tissue section with cells 1–4 marked by white circles (bar = 6 μm). (B) MS locus ABI20 was analyzed by PCR amplification and capillary electrophoresis. Both paternal and maternal alleles are visible in the tail clipping sample (Tail) and in cell 3. Allelic dropout (ADO) can be seen in the short allele of cells 1, 2, and 4. Slippage mutations (arrows) can be seen in the long allele of cells 2 and 4. The negative control (NC) sample shows no amplification. (C) Sequencing of exon 8 from the P53 gene. A point mutation (C => T) can be seen in the 18th nucleotide from the left (highlighted grey) in cells 1 and 2. In cell 3, both the normal and mutated alleles were amplified, and in cell 4 only a normal allele is visible. (D) Mitochondrial ND3 gene locus was amplified by PCR and run on an agarose gel. Amplification is successful for all cells from a fresh section, but not for all cells from a pre-stored section.