Figure 3

ELISA experiment showing reactivity of three different sources of p53 antigen to various commercial antibodies. The purified (His)6-p53 fusion protein 1 μg/ml (A) and (His)6-p53 fusion protein 100 μg/ml directly immobilized onto nickel coated microtiter plate (B) were subjected to react with various amounts of anti-p53 mAb (0, 1:10000, 1:5000 and 1:1000 dilution) and anti-histidine mAb (1:1000 dilution). The p53-BCCP fusion protein directly immobilized onto avidin-coated microtiter plate were reacted with various amounts of anti-p53 mAb (0, 1:10,000, 1:5,000 and 1:1,000 dilution) and anti-biotin mAb (1:1000 dilution). Experiment was performed in triplicate and error bars represent standard deviation.