Solubility enhancement of aggregation-prone heterologous proteins by fusion expression using stress-responsive Escherichia coliprotein, RpoS

Table 3 Solubility of the expressed recombinant proteins

Heterologous proteins	Solubility* of the expressed recombinant proteins (%)
	RpoS-fusion expression	GST-fusion expression	Direct expression
G-CSF	87.4 ± 2.1	91.5 ± 1.5	4.6 ± 0.5
CUT	44.9 ± 2.7	26.2 ± 1.1	3.1 ± 0.2
AID	18.1 ± 3.5	41.7 ± 1.5	8.7 ± 1.6
hFTN-L	47.8 ± 2.1	87.6 ± 2.8	1.7 ± 0.4
NACHT	36.4 ± 4.3	74.8 ± 2.2	8.4 ± 1.3
GAD_448–585	59.0 ± 1.8	7.8 ± 2.7	1.9 ± 0.5
hIL-2	60.1 ± 2.4	85.7 ± 1.7	1.3 ± 0.3
EGF	80.4 ± 0.9	92.9 ± 1.2	3.0 ± 0.9
ppGRN	89.2 ± 3.5	90.1 ± 2.3	7.6 ± 0.6
mp-INS	75.9 ± 2.8	13.3 ± 1.0	1.3 ± 0.4

* The solubility was defined as the fraction of the soluble recombinant protein compared to the synthesized total (soluble + insoluble) recombinant protein. Average and standard deviation values were calculated based on the results of repeated triplicate experiments.

ISSN: 1472-6750