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Table 2 Primers used for the cloning of genes encoding various heterologous proteins

From: Solubility enhancement of aggregation-prone heterologous proteins by fusion expression using stress-responsive Escherichia coliprotein, RpoS

Heterologous proteins

Primer sequences

  

Direct expression

Fusion expression

mp-INS

Sense

cat atg ttt gtc aac caa cat

ctc gag ttt gtc aac caa cat

 

Antisense

aag ctt tta gtt aca gta gtt c

aag ctt tta gtt aca gta gtt c

EGF

Sense

cat atg aac tct gac tcc gaa tgc

ctc gag aac tct gac tcc gaa tgc

 

Antisense

aag ctt tta acg cag ttc cca cca

aag ctt tta acg cag ttc cca cca

ppGRN

Sense

cat atg ggc tcc agc ttc ctg

ctc gag ggc tcc agc ttc ctg

 

Antisense

aag ctt tca ctt gtc ggc t

aag ctt tca ctt gtc ggc t

hIL-2

Sense

cat atg gca cct act tca agt

ctc gag gca cct act tca agt

 

Antisense

aag ctt tta tca agt cag tgt

aag ctt tta tca agt cag tgt

AID

Sense

cat atg gac agc ctc ttg atg aac

ctc gag gac agc ctc ttg atg aac

 

Antisense

aag ctt tca taa caa aag tcc ca

aag ctt tca taa caa aag tcc ca

GAD448–585

Sense

cat atg cgc cac gtt gat gt

ctc gag cgc cac gtt gat gt

 

Antisense

atc gat tta taa atc ttg tcc

atc gat tta taa atc ttg tcc

CUT

Sense

cat atg gct ccc ctg ccg gat ac

ctc gag gct ccc ctg ccg gat ac

 

Antisense

aag ctt tta aag ccc gcg gcg ct

aag ctt tta aag ccc gcg gcg ct

   

aag ctt atg atg gtg gtg atg atg tta aag ccc gcg gcg ct (for metal affinity purification)

hFTN-L

Sense

cat atg agc tcc cag att cgt

ctc gag agc tcc cag att cgt

 

Antisense

aag ctt tta gtc gtg ctt gag agt

aag ctt tta gtc gtg ctt gag agt

G-CSF

Sense

cat atg act cca ctc gga cct g

ctc gag acc ccc ctg ggc cct gcc

   

ctc gag gac gat gac gat aaa acc ccc ctg ggc cct gcc (for enterokinase digestion)

 

Antisense

aag ctt tca tgg ctg tgc aag

aag ctt tca tgg ctg tgc aag

NACHT

Sense

cat atg act gtg gtg ttc cag

ctc gag act gtg gtg ttc cag

 

Antisense

aag ctt tca cag cag gta gta c

aag ctt tca cag cag gta gta c