Solubility enhancement of aggregation-prone heterologous proteins by fusion expression using stress-responsive Escherichia coliprotein, RpoS

Table 1 Result of proteome-wide finding of some aggregation-resistant E. coli proteins

Gene name^a	Access No.^a	Protein name^a	pI/MW(kDa)		Sequence coverage^d	Score^d	Fold change
			Theoretical^b	Experimental^c
rpoS	P13445	RNA polymerase sigma factor rpoS	4.89/37.97	5.08/38.95	19	36	6.21
groL	P0A6F5	HSP60 chaperonin	4.85/57.20	4.90/58.63	16	34	2.13
htpG	P0A6Z3	Chaperone protein htpG	5.09/71.38	5.14/66.37	9	27	3.10
nusA	P0AFF6	Transcription elongationprotein nusA	4.53/54.87	4.69/59.09	16	44	3.46
gyrA	P0AES4	DNA gyrase subunit A	5.09/96.96	5.20/92.23	21	29	1.95
pflB	P09373	Formate acetyltransferase 1	5.69/85.23	5.68/83.51	19	39	3.26
sdhA	P0AC41	Succinate dehydrogenase flavoprotein subunit	5.85/64.42	5.70/65.73	25	43	2.16

^a Gene name, accession number, and protein name were obtained from ExPASy Proteomics Server [35].
^b Theoretical values of pI and Mw were calculated using "Compute pI/Mw tool" [36].
^c Experimental values of pI and Mw were estimated through 2-DE gel image analysis in the present study.
^d Sequence coverage and score values were calculated using "ALDENTE : PEPTIDE MASS FINGERPRINTING TOOL" [37].

ISSN: 1472-6750