Figure 5From: Solubility enhancement of aggregation-prone heterologous proteins by fusion expression using stress-responsive Escherichia coliprotein, RpoSBioactivity of recombinant fusion mutant, RpoS::CUT. Assay results using cell-free supernatants from (A) E. coli BL21 (DE3) host and (B) recombinant E. coli BL21 (DE3) [pT7-RpoS-CUT] producing RpoS::CUT. Both PNB (●) and PNP (○) were used as substrates for the cutinase activity assay. (Concentrations: PNB = 6.6 mM; PNP = 6.6 mM).Back to article page