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Figure 2 | BMC Biotechnology

Figure 2

From: Solubility enhancement of aggregation-prone heterologous proteins by fusion expression using stress-responsive Escherichia coliprotein, RpoS

Figure 2

The plasmid vector constructions for direct and fusion expression of heterologous proteins in E. coli. (A) Direct expression vector, (B) RpoS- and GST-fusion expression vector, (C) Hybrid vector for metal (Ni+2) affinity purification of RpoS-CUT-(His)6, (D) Hybrid vector for metal (Ni+2) affinity purification of (His)6-RpoS-D4K-G-CSF, followed by enterokinase cleavage.

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BMC Biotechnology

ISSN: 1472-6750

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