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Table 4 Characterization of the recombinant Bm86, Bd86 and Ba86 purification process.

From: Expression of recombinant Rhipicephalus (Boophilus) microplus, R. annulatus and R. decoloratus Bm86 orthologs as secreted proteins in Pichia pastoris

Purification stages Bm86 Bd86 Ba86
  Total protein conc. (mg·L-1) Rec. protein conc. (mg·L-1) Purity (%) Recovery (%) Total protein conc. (mg·L-1) Rec. protein conc. (mg·L-1) Purity (%) Recovery (%) Total protein conc. (mg·L-1) Rec. protein conc. (mg·L-1) Purity (%) Recovery (%)
Fermentation supernatant 274 150 55 --- 194 110 57 --- 170 112 66 ---
Culture separation and microfiltration 137 96 70 60 84 63 75 55 99 77 78 62
Ultrafiltration and diafiltration 407 326 80 35 451 370 82 42 370 314 85 40
  1. Abbreviations: conc., concentration; rec., recombinant.