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Table 2 Screening for Bm86, Bd86 and Ba86 expression in the culture supernatant of P. pastoris transformants.

From: Expression of recombinant Rhipicephalus (Boophilus) microplus, R. annulatus and R. decoloratus Bm86 orthologs as secreted proteins in Pichia pastoris

Recombinant strain Parental strain Recombinant protein Total protein concentration (mg·L-1)a Recombinant protein concentration (mg·L-1)b % of total proteinc
GS115Moz9-1 GS115 Bm86 66.5 3.0 4.5
GS115Moz9-2* GS115 Bm86 65.5 3.3 5.0
GS115Moz9-3 GS115 Bm86 65.3 1.0 1.5
KM71HMoz9-1 KM71H Bm86 66.3 1.0 1.5
KM71HMoz9-2 KM71H Bm86 66.8 3.0 4.5
KM71HMoz9-3 KM71H Bm86 64.8 1.5 2.3
GS115Dec8-1 GS115 Bd86 64.4 1.0 1.6
GS115Dec8-2 GS115 Bd86 66.4 1.5 2.3
GS115Dec8-3 GS115 Bd86 66.0 1.5 2.3
KM71HDec8-1* KM71H Bd86 66.0 2.0 3.0
KM71HDec8-2 KM71H Bd86 63.4 1.5 2.4
KM71HDec8-3 KM71H Bd86 63.5 1.0 1.6
X33pBaI 1 X33 Ba86 49.7 1.0 2.0
X33pBaI 2 X33 Ba86 45.5 1.0 2.2
X33pBaI 3* X33 Ba86 45.4 6.0 13.2
X33pBaII 1 X33 Ba86 55.8 5.5 9.8
X33pBaII 2 X33 Ba86 48.3 5.0 10.4
X33pBaII 3 X33 Ba86 46.9 4.0 8.5
  1. The experiments were conducted twice with similar results.
  2. aDetermined using the Bradford method with BSA as standard [49].
  3. bDetermined by semi-quantitative analysis in dot-blots using a standard curve constructed with known amounts of recombinant Bm86 extracted from Gavac (Revetmex).
  4. cDetermined as the percent of recombinant protein in total preoteins.
  5. *Recombinant strains with highest Bm86, Bd86 and Ba86 concentration in the culture supernatant were selected for fermentation and protein production.