Immune cross-reactivity between Bm86 ortholog proteins. Western blot analysis of the purified recombinant Ba86 (lane 1), Bd86 (lane 2) and Bm86 (lane 3) proteins. On each well 1.5 μg proteins were loaded. Membranes were probed with serum from rabbitts immunized with recombinant Ba86 (A), Bd86 (B) and Bm86 (C) diluted 1:5000. Membranes were washed three times with TBS and incubated with an anti-rabbit IgG HRP conjugate (Sigma-Aldrich) diluted 1:1000 in TBS. After washing the membrane again, color were developed using TMB stabilized substrate for HRP (Promega). MW: molecular weight marker (ColorBurst, Sigma-Aldrich). The position of recombinant proteins is indicated with arrows.