Characterization of purified recombinant proteins. Western blot analysis of the purified recombinant Bm86 (lane 2), Bd86 (lane 3) and Ba86 (lane 4) proteins. On each well, 3.5 μg proteins were loaded. Membranes were probed with serum from rabbits immunized with control Bm86 (Gavac; Revetmex) diluted 1:1000. Membranes were then washed three times with TBS and incubated with an anti-rabbit IgG HRP conjugate (Sigma-Aldrich) diluted 1:1000 in TBS. After washing the membranes again, color was developed using TMB stabilized substrate for HRP (Promega). Lane 1: molecular weight markers (MW; ColorBurst, Sigma-Aldrich). The position of recombinant proteins is indicated with arrows.