Figure 4

Representative gel view and MCA using sex-specific primers of S. c. hoya. PCR products with 185- and 112-bp amplified by (A) CHD-W-F/R and (B) CHD-Z-F/R primers in different PCR-wells were run in 4% agarose gel, respectively. The arrow of Fig. 4A indicates the remaining primers or primer-dimers of PCR. Non-specific products of W-185 indicated with star symbols were only occurred in male samples. (C, D) demonstration for high-throughput molecular sexing of multiple S. c. hoya samples using MCA. All data are performed in duplicate. (C) 7 females (Bds 12, 14, 15, 17, 19, 20, and 23) and (D) 6 males (Bds 13, 16, 18, 21, 22, and 24) were included. Z-112 and W-185 represent the primer mixtures for CHD-Z-F/CHD-Z-R (112-bp) and CHD-W-F/CHD-W-R (185-bp). The Tm value for Z-112 and W-185 is 75.0°C and 79°C, respectively. The Tm of non-specific products of W-185 is greatly larger than 79°C.