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Figure 3 | BMC Biotechnology

Figure 3

From: High-throughput avian molecular sexing by SYBR green-based real-time PCR combined with melting curve analysis

Figure 3

Real-time PCR/MCA using primer sets P2/P8. DNAs were chosen from S. c. hoya sample Bd6 (A, B) and P. sinensis sample 3424 (C, D), which are the ecology female control. (A, C) Real-time PCR data. (B, D) Data of MCA. The Tm of P2/P8 PCR products of S. c. hoya and P. sinensis is about 80.0 and 80.5°C, respectively. These data are collected from duplicated experiments. RFU, Relative fluorescence unit.

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