Figure 3
From: Quantification of PRL/Stat5 signaling with a novel pGL4-CISH reporter
Expression of pGL4-CISH to PRL and PRLr in different human breast cancer and epithelial cell lines. Cells were transfected with pGL4-CISH. After transfection, cells were arrested in minimal defined medium without FBS, the transfectants were stimulated with PRL for 24 hours prior to analysis of luminescence. A. Cells lines were transfected with 50 ng pGL4-CISH/1 × 105 cells. B. MCF7 cells were overexpressed with pEF-PRLr and treated with PRL (200 ng/ml). *, P < 0.05. ***, P < 0.001. C. The low inset is Western blot demonstrating PRLr overexpression level obtained with the various transfectants with the arrows indicating each lane of treatments. Error bars represent SEM; * denotes P < 0.05, and *** denotes P < 0.001 as compared with no PRL stimulation alone.