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Figure 7 | BMC Biotechnology

Figure 7

From: Improved RNA quality and TaqMan®Pre-amplification method (PreAmp) to enhance expression analysis from formalin fixed paraffin embedded (FFPE) materials

Figure 7

Analysis of GAPDH using two sizes of amplicon showing the difference of C T s between FFPE and snap frozen counterparts. ΔCT = CT (FFPE) - CT (Snap-Frozen). The smaller amplicon size of GAPDH (67 bp) generated lower ΔCTs than the larger amplicon size of 122 bp indicating that smaller amplicon sizes produced more comparable CTs in snap frozen and FFPE samples.

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BMC Biotechnology

ISSN: 1472-6750

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