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Figure 2 | BMC Biotechnology

Figure 2

From: Improved RNA quality and TaqMan®Pre-amplification method (PreAmp) to enhance expression analysis from formalin fixed paraffin embedded (FFPE) materials

Figure 2

Qualities of RNA were measured using Agilent 2100 Bioanalyzer. (Str = Stratagene protocol; Am = Ambion protocol; + = modified with incubation in Proteinase K buffer at 70°C for 20 minutes; and SN = snap frozen.) Modification to the Stratagene and Ambion protocols generated larger fragments (lane 2 and 5) compared to the extracts generated using the original protocols (lane 1 and 4). Each well contained 1 μl of extracted RNA from equal amount of starting materials of 105 cells.

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BMC Biotechnology

ISSN: 1472-6750

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