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Figure 2 | BMC Biotechnology

Figure 2

From: Production of highly knotted DNA by means of cosmid circularization inside phage capsids

Figure 2

Packaging efficiency and knotting probability of cosmid DNA in P4 phage particles. DNA was extracted from either no cosmid (a), P4cos-8 (b), P4cos-5 (c) and P4cos-3 (d). Samples were loaded in the gel before and after unknotting the DNA with topoisomerase II (lanes 1 and 2, respectivelly, in a-d). Gel electrophoresis were done in 0.6% agarose gels with TBE buffer (1.4 volt/cm × 30 hours) in a and b; and in 0.8% agarose gels with TBE buffer (1.6 volt/cm × 20 hours) in c and d. Lambda DNA digested with HindIII was loaded in lane 0 in b, c, d. Nicked-circular (CCOS) and linear (LCOS) forms of each corresponding cosmid, produced by restriction endonuclease treatment of the supercoiled plasmids, were loaded as markers in lane 3 in b, c, d. Positions of the unknotted nicked-circle (CP4) and the linear form (LP4) of P4 DNA are indicated.

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