Subcellular localization of expressed hERG. Representative confocal microscopy images of cells fixed, permeablised and immunolabelled with anti-hERG antibody C20. CHO and CHO hERG cells grown at 37°C were split and kept subconfluent for 3 d at 37 (A,B), 30 (C), or 27°C (D). Additional samples were kept at 30°C for 1 d, followed by 24 h incubation at the same temperature with 1 μM thapsigargin (E). Bar, 5 μm.