Comparison of GFP, immunostaining and FISH signals without RNAse digestion, allowing for detection of RNA from the transgene arrays by the FISH probe. For panel labeling see Figure 2. Projections of deconvolved images are shown. Note the larger volume of FISH signals compared to other signals. In this experiment, due to the large differences in signal appearance, pre- and post-FISH signals could not be subjected to automated image processing. Therefore, for this experiment only, post-FISH images were matched to the orientation of pre-FISH images manually.