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Figure 6 | BMC Biotechnology

Figure 6

From: Plasmid-based genetic modification of human bone marrow-derived stromal cells: analysis of cell survival and transgene expression after transplantation in rat spinal cord

Figure 6

Transgene expression of subcutaneous hMSC-NT3-EGFP cell transplants. Histological assessment of EGFP expression by subcutaneous hMSC-NT3-EGFP cell transplants at three time points post transplantation (day 1, week 1 and week 2). First row: hematoxylin-eosin (HE) staining indicating the presence of nucleated cells into the subcutaneously transplanted matrigel. Second row: direct EGFP fluorescence indicating the presence of EGFP positive hMSC-NT3-EGFP cell transplants. Third row: immuno-histochemical staining for EGFP indicating the presence of EGFP positive hMSC-NT3-EGFP cell transplants. Fourth row: immuno-histochemical staining for human mitochondrial antigen (hMA) indicating the human nature of the observed EGFP positive hMSC-NT3-EGFP cell transplants. Representative pictures were chosen from multiple subcutaneous hMSC-NT3-EGFP cell transplants analysed 1 day (n = 6), 1 week (n = 5) and 2 weeks (n = 3) post-transplantation.

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BMC Biotechnology

ISSN: 1472-6750

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