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Figure 5 | BMC Biotechnology

Figure 5

From: Plasmid-based genetic modification of human bone marrow-derived stromal cells: analysis of cell survival and transgene expression after transplantation in rat spinal cord

Figure 5

Analysis of transgene expression by hMSC-NT3-EGFP cell transplants following laser capture micro-dissection (LCM). (A) Laser Capture Micro-dissection (LCM) of hMSC-NT3-EGFP cell transplants at day 1 and day 7 post-transplantation in rat spinal cord. Pictures showing cresyl-violet stained slides pre-and post-LCM. (B) Upper graph showing the level of transgenic EGFP mRNA transcripts/1000 copies GAPDH in hMSC-EGFP cell cultures (CELLS, n = 1), on transplanted hMSC-EGFP cells at day 1 post-transplantation (DAY 1, n = 1), and on transplanted hMSC-EGFP cells at day 7 post-transplantation (DAY 7, n = 1). Lower graph showing the level of transgenic EGFP mRNA transcripts/1000 copies GAPDH in hMSC-NT3-EGFP cell cultures (CELLS, n = 1), on transplanted hMSC-NT3-EGFP cells at day 1 post-transplantation (DAY 1, n = 1), and on transplanted hMSC-NT3-EGFP cells at day 7 post-transplantation (DAY 7, n = 1).

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BMC Biotechnology

ISSN: 1472-6750

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