Transgene expression of hMSC-NT3-EGFP cell transplants in rat spinal cord. (A) Histological assessment of EGFP expression by hMSC-NT3-EGFP cell transplants in rat spinal cord at three time points post transplantation (day 1, week 1 and week 2). First row: hematoxylin-eosin (HE) staining indicating localisation and general appearance of transplantation site. Second row: immuno-histochemical staining for EGFP showing a decrease in EGFP expression over time by hMSC-NT3-EGFP cell transplants. Third row: immuno-histochemical staining for human mitochondrial antigen (hMA) indicating the human nature of the hMSC-NT3-EGFP cell transplants. Representative pictures were chosen from multiple hMSC-NT3-EGFP cell-transplanted spinal cords analysed 1 day (n = 7), 1 week (n = 11) and 2 weeks (n = 4) post-transplantation. (B) Detail image of the pictures described in part (A) of this figure.