TY - JOUR AU - Ronsyn, Mark W. AU - Daans, Jasmijn AU - Spaepen, Gie AU - Chatterjee, Shyama AU - Vermeulen, Katrien AU - D'Haese, Patrick AU - Van Tendeloo, Viggo FI AU - Van Marck, Eric AU - Ysebaert, Dirk AU - Berneman, Zwi N. AU - Jorens, Philippe G. AU - Ponsaerts, Peter PY - 2007 DA - 2007/12/14 TI - Plasmid-based genetic modification of human bone marrow-derived stromal cells: analysis of cell survival and transgene expression after transplantation in rat spinal cord JO - BMC Biotechnology SP - 90 VL - 7 IS - 1 AB - Bone marrow-derived stromal cells (MSC) are attractive targets for ex vivo cell and gene therapy. In this context, we investigated the feasibility of a plasmid-based strategy for genetic modification of human (h)MSC with enhanced green fluorescent protein (EGFP) and neurotrophin (NT)3. Three genetically modified hMSC lines (EGFP, NT3, NT3-EGFP) were established and used to study cell survival and transgene expression following transplantation in rat spinal cord. SN - 1472-6750 UR - https://doi.org/10.1186/1472-6750-7-90 DO - 10.1186/1472-6750-7-90 ID - Ronsyn2007 ER -