Structure of the Ad/GFP
vector and controls. All transgene cassettes were first cloned into pLAd and pRAd shuttle vectors as described in the Methods section. Vector genomes were then assembled in vitro, as described previously [30, 31]. The resulting rAd vectors are E1-deleted and have a deletion in the E3 region (E3 promoter is retained). They also lack all of the E4 ORFs, except orf6, which is expressed from the E4 promoter.