Effect of L-glutamine supplementation on Gadd153 expression in batch cultures of Bcl-xL-transfected Sp2/0 cells. Cells were processed for stationary batch cultures and supplemented with either PBS (panels C and E) or L-glutamine (Panels D and F) on culture day 4 (pTEJ8-Sp2/0) or day 5 (Bcl-xL-Sp2/0). A) Viable cell density. B) Percent viable cells. C and D) Immunoblot analysis of Gadd153 expression in pTEJ8-Sp2/0. E and F) Immunoblot analysis of Gadd153 in batch cultures of the Bcl-xL-transfected Sp2/0 cells. For panels A and B, data is the average ± standard deviation of three independent experiments. * = p < 0.01 vs pTEJ8 control. ¶= p < 0.05 vs PBS-supplemented control. Yellow bars: Sp2/0-pTEJ8 cells treated with PBS. Light blue bars: Sp2/0-pTEJ8 cells supplemented with L-glutamine. Dark blue bars: Sp2/0-BclxL cells supplemented with PBS. Dark red bars: Sp2/0-Bcl-xL cells treated with L-glutamine. Immunoblotting data are representative of 3 independent experiments.