Gadd153 expression in batch cultures of Bcl-xL-transfected Sp2/0 cells. A) Immunoblot analysis of Bcl-xL expression in wild type Sp2/0, pTEJ8-Sp2/0 and Bcl-xL-Sp2/0 cells. B) Viable cell density. C) Percentage of viable cells in the culture. D) Immunoblot analysis of Gadd153 in batch cultures of the pTEJ8-transfected Sp2/0 cells. E) Immunoblot analysis of Gadd153 in batch cultures of the Bcl-xL-transfected Sp2/0 cells. Data in panels B and C are expressed as the average ± standard deviation of 3 independent experiments. Data in panels D and E are representative of 3 independent experiments. Open symbols: Bcl-xL-transfected cells. Closed symbols: vector-transfected control.