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Figure 2 | BMC Biotechnology

Figure 2

From: GADD153 expression does not necessarily correlate with changes in culture behavior of hybridoma cells

Figure 2

Effect of L-glutamine supplementation on the expression of Gadd153. Sp2/0 cells were processed for batch culture and supplemented on culture day 4 with either PBS (closed symbols) or L-glutamine (final concentration: 4 mM. Open symbols.). A) Viable cell density. B) Percentage of viable cells in the culture. C) Semi-quantitative RT-PCR analysis of Gadd153 mRNA levels. Numbers at the bottom of the figure indicate the number of amplification cycles. D) Immunoblot analysis of Gadd153 expression in the PBS-treated control. E) Immunoblot analysis of Gadd153 expression in the L-glutamine-supplemented culture. Data in panels A and B are expressed as the average ± standard deviation of 3 independent experiments. Data in panels C-E are representative of 3 independent experiments.

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