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Figure 2 | BMC Biotechnology

Figure 2

From: Arginine-to-lysine substitutions influence recombinant horseradish peroxidase stability and immobilisation effectiveness

Figure 2

Scanned images of DAB-stained, spotted immobilised rHRP activity. WT, Wildtype; Mutant 1, R118K/R159K/R283K; Mutant 2, R118K/R159K; Mutant 3, R118K/R159K/K232N/K241F/R283K; Mutant 4, R118K/R159K/K232N/K241F. Time points indicated on the left are in hours. DAB coloration indicates peroxidase activity; this was noted within minutes for the immobilisation mutants, whereas plant and wildtype recombinant HRP required a longer time to develop any coloration.

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BMC Biotechnology

ISSN: 1472-6750

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