Figure 5

Schematic representation of the « dual color/selection » PRIG vectors. pPRICp eYFPHA is a pPRICp aHA derivative in which the eYFP sequence has been cloned upstream of the MCS. pPRIGp mChHA is a pPRIGp aHA derivative in which mCherry sequence has been cloned upstream of the MCS. pPRIPu eGFP is a pPRIPu derivative in which the eGFP sequence has been cloned upstream of the MCS. The reading frame is open from the start codon of the upstream fluorescent protein to the stop codon of the HA sequence (for pPRICp eYFPHA and pPRIGp mChHA) or to the in-frame stop codon 3' to the MCS (for pPRIPu eGFP). pPRICp eYFPHA and pPRIGp mChHA, but not pPRIPu eGFP, harbor the two flanking PacI sites. For each vector, the unique sites of the MCS are indicated above the MCS.