Figure 2

Schematic representation of the HA3' PRIG vectors. pPRIGp a/b/c HA are three pPRIG derivatives containing a HA tag sequence downstream of the MCS. Each of these derivatives harbors the HA sequence in one of the three reading frames relative to the MCS (hence pPRIG a/b/cHA). The reading frame is therefore open from and throughout the MCS to the stop codon located at the 3' end of the HA sequence. Moreover, the IRES-eGFP cassette is flanked by two PacI sites (Pa) a very rare cutter, hence pPRIGpa/b/c HA, allowing for accurate removal of the cassette. For each vector, the unique sites of the MCS are indicated above the MCS.