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Figure 1 | BMC Biotechnology

Figure 1

From: A new generation of pPRIG-based retroviral vectors

Figure 1

Schematic representation of the pAP2 and pPRIG vectors. The parental pAP2 retroviral vector harbors the CMV enhancer/promoter (CMVpro) in place of the U3 region of the 5' LTR and eGFP downstream of an ECMV-derived IRES containing two point mutations (hence indicated as IRES*) which strongly weakens its activity. The pPRIG HAa/b/c vectors improve pAP2 by: 1) replacing IRES* by the completely wild-type ECMV-derived IRES sequence (IRES), which restores its full activity; 2) inserting a much more complete multicloning site (MCS) and T7 and SP6 phage promoter on each side of the new MCS; and 3) adding a HA tag sequence in the three reading frames (a/b/c) 5' of the MCS. pPRIG HAa/b/c, as well as a pPRIG (without HA), were previously described and are used here to generate new vectors. For each vector, the unique sites of the MCS are indicated above the MCS.

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